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Additional complications come from the burning of fossil fuels such as coal and oil, and from the above-ground nuclear tests done in the 1950s and 1960s.Because the time it takes to convert biological materials to fossil fuels is substantially longer than the time it takes for its in the atmosphere, which attained a maximum in 1963 of almost twice what it had been before the testing began.They synthesized Libby and several collaborators proceeded to experiment with methane collected from sewage works in Baltimore, and after isotopically enriching their samples they were able to demonstrate that they contained radioactive .By contrast, methane created from petroleum showed no radiocarbon activity because of its age.The resulting data, in the form of a calibration curve, is now used to convert a given measurement of radiocarbon in a sample into an estimate of the sample's calendar age.Other corrections must be made to account for the proportion of throughout the biosphere (reservoir effects).In addition to permitting more accurate dating within archaeological sites than previous methods, it allows comparison of dates of events across great distances.

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The resulting radiocarbon combines with atmospheric oxygen to form radioactive carbon dioxide, which is incorporated into plants by photosynthesis; animals then acquire in a sample from a dead plant or animal such as a piece of wood or a fragment of bone provides information that can be used to calculate when the animal or plant died.

The older a sample is, the less (the period of time after which half of a given sample will have decayed) is about 5,730 years, the oldest dates that can be reliably measured by this process date to around 50,000 years ago, although special preparation methods occasionally permit accurate analysis of older samples.

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